Failure of maleic hydrazide to act as a sulfhydryl or carbonyl reagent.

Paper Chromatography of Steroids

In Paper I of this series (1) a method was described for the paper chromatographic analysis of certain ketonic steroids as hydrazones formed with Girard’s Reagent T. When that technique was applied to the adrenal cortical hormones, however, certain deficiencies appeared. (a) The RI values of the individual steroid-Reagent T hydrazones were not sufficiently different to permit identification. (b...

The effects of maleic hydrazide on growth control of perennial ryegrass (lolium perenne L.CV. yarandi)

Factors affecting the formation of gum by Alcaligenes faecalis.

Maleic hydrazide (1, 2-dihydropyridazine-3, 6dione) is an organic plant growth regulator which has found use in such varied areas as prevention of sucker formation in tobacco, prevention of sprouting of some stored vegetables, and as a herbicide. In commercial application the diethanolamine salt (diethanolammonium maleic hydrazide or diethanolammonium-1 ,2-dihydropyridazine-3, 6-dione) is often...

Staining of blood cells with periodic acid/salicyloyl hydrazide (PA-SH). A fluorescent method for demonstrating glycogen.

By J. BURNS AND P. B. NEAME T lIE PERIODIC ACID-SCHIFF (PAS ) REACTION is generally accepted as the method of choice for demonstrating glycogen in blood cells. This reaction depends upon the presence, in glycogen, of vicinal glycols which are oxidized by periodic acid to dialdehydes. The latter are subsequently condensed with Schiff’s reagent to produce a magenta-red product, visible by light m...

Ability of Allium cepa L. root tips and Tradescantia pallida var. purpurea in N-nitrosodiethylamine genotoxicity and mutagenicity evaluation

N -nitroso compounds, such as N -nitrosodiethylamine (NDEA), can be formed by the reaction of secundary amines with nitrosating agents, and are suspected to be involved in tumors in humans. NDEA has been considered a weak carcinogen in genotoxic assays probably due to the inefficient nitrosamine activation system that is used and/or to the efficient repair system. In this work, we evaluated the...